{"id":591,"date":"2020-03-13T09:08:03","date_gmt":"2020-03-13T09:08:03","guid":{"rendered":"https:\/\/corakid.com\/?page_id=591"},"modified":"2020-03-13T09:18:39","modified_gmt":"2020-03-13T09:18:39","slug":"sequencage-ngs","status":"publish","type":"page","link":"https:\/\/corakid.com\/en\/core-facilities\/genomique-et-biologie-cellulaire\/sequencage-ngs\/","title":{"rendered":"S\u00e9quen\u00e7age NGS"},"content":{"rendered":"

Bioanalyseur 2100 Agilent<\/strong><\/p>\n\n\n\n

Le Bioanalyseur permet de mesurer la taille et de quantifier les ARN, ADN et prot\u00e9ines. Il n\u00e9cessite une petite quantit\u00e9 (1\u00b5L) d\u2019\u00e9chantillon et donne des r\u00e9sultats fiables et rapides. Il est essentiel pour v\u00e9rifier la qualit\u00e9 des librairies avant s\u00e9quen\u00e7age. <\/p>\n\n\n\n

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\"\"<\/figure>\n<\/div>\n<\/div>\n\n\n\n

MiSeq illumina\u00a0: s\u00e9quenceur moyen d\u00e9bit NGS<\/strong><\/p>\n\n\n\n

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Le Miseq permet de g\u00e9n\u00e9rer 25 millions de s\u00e9quences maximum. Les lectures peuvent aller jusqu’\u00e0 600 nucl\u00e9otides en paired-end (2X300), soit un maximum de 15 Gigabases lues. Il peut r\u00e9pondre \u00e0 des besoins vari\u00e9s comme la capture de s\u00e9quences cibles (design \u00e0 fa\u00e7on), du \u00ab\u00a0small genome\u00a0\u00bb et \u00ab\u00a0small RNA\u00a0\u00bb, du res\u00e9quen\u00e7age et du s\u00e9quen\u00e7age\u00a0\u00a0 d\u2019amplicons. <\/p>\n<\/div><\/div>\n\n\n\n

Prestations<\/strong> : <\/p>\n\n\n\n

  • Dosage (Qubit) et V\u00e9rification de la qualit\u00e9 des\nADNs par Bioanalyseur <\/li>
  • Design des sondes pour les exp\u00e9riences de capture<\/li>
  • Pr\u00e9paration des librairies (capture de s\u00e9quences\ncibles par le kit NimbleGen Roche)<\/li>
  • Passage sur Miseq et v\u00e9rification de la qualit\u00e9\ndes runs logiciel FastQC<\/li>
  • Rendu des donn\u00e9es (fichiers fastq) et sur demande\nfichiers vcf et bam.<\/li><\/ul>","protected":false},"excerpt":{"rendered":"

    Bioanalyseur 2100 Agilent Le Bioanalyseur permet de mesurer la taille et de quantifier les ARN, ADN et prot\u00e9ines. Il n\u00e9cessite une petite quantit\u00e9 (1\u00b5L) d\u2019\u00e9chantillon et donne des r\u00e9sultats fiables et rapides. Il est essentiel pour v\u00e9rifier la qualit\u00e9 des librairies avant s\u00e9quen\u00e7age. MiSeq illumina\u00a0: s\u00e9quenceur moyen d\u00e9bit NGS Le Miseq permet de g\u00e9n\u00e9rer 25 […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":233,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_cbd_carousel_blocks":"[]","footnotes":""},"class_list":["post-591","page","type-page","status-publish","hentry"],"yoast_head":"\nS\u00e9quen\u00e7age NGS - CoRaKiD<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/corakid.com\/en\/core-facilities\/genomique-et-biologie-cellulaire\/sequencage-ngs\/\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"S\u00e9quen\u00e7age NGS - CoRaKiD\" \/>\n<meta property=\"og:description\" content=\"Bioanalyseur 2100 Agilent Le Bioanalyseur permet de mesurer la taille et de quantifier les ARN, ADN et prot\u00e9ines. Il n\u00e9cessite une petite quantit\u00e9 (1\u00b5L) d\u2019\u00e9chantillon et donne des r\u00e9sultats fiables et rapides. Il est essentiel pour v\u00e9rifier la qualit\u00e9 des librairies avant s\u00e9quen\u00e7age. 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